Single Particle Tracker with total internal reflection fluorescence (TIRF) microscopy for multi-color single-particle tracking, co-localization and high-content screening.
Key Features:
- TIRF/HILO imaging in vitro or in vivo, allowing single particle localization and tracking in real-time
- Microinjection platform for fast, reliable in situ transfection
- Two Andor EMCCD cameras for high sensitivity multi-color imaging
- Astigmatic super-resolution axial localization
- Zero-drift compensation for long observations
- Imaris particle-tracking software
Components:
- IX-81 computer-controlled Olympus microscope body with 100X oil 1.4NA (UPLSAPO100XO) and 60X oil 1.49NA (APON60XOTIRFM); quadband dichroics (Chroma) 405/488/532/640 and 405/488/561/640 nm excitation bands
- Cell^TIRF (Olympus) module including independent focusing of four fiber inputs
- Five lasers modulated and fiber coupled (Coherent CUBE 405-100, CUBE 488-50, CUBE 640-100, Sapphire 561-50 CW and Crystalaser CL532-150mW-L)
- P-545.3C7 capacitive piezoelectric x-y-z stage (Physik Instrumente) with very high long-term stability
- Tucam unit (Andor) for 2-color full-frame imaging on two Andor iXon Ultra EM-CCD cameras
- Metamorph (Molecular Devices) computer interface to control all hardware components
