Optical Tweezers + Fluorescence Microscope
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The SPN is a custom-built optical tweezer system which also incorporates TIRF/epifluorescence imaging. Optical tweezers allow measurement and manipulation of biomolecules at forces and displacements at scales relevant to single biomolecules and cells: down to sub-pN and sub-nm.
Example Applications
- Motor protein force/displacement measurement
- Nucleoprotein force measurement
- Phase separation characterization
- Cellular stiffness measurement
Key Features
- Dual-beam optical traps
- Independent trapping and detection lasers allow fast, easy calibration of every bead
- Widefield/TIRF illumination with 405nm, 532nm, and 640nm laser lines
- Dual-view EMCCD fluorescent imaging
