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  3. BIOPHYSICS SEMINAR<br>Evanescence in Fluorescence Excitation and Emission Microscopy
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BIOPHYSICS SEMINAR<br>Evanescence in Fluorescence Excitation and Emission Microscopy

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        1. <b>BIOPHYSICS SEMINAR</b><br>Electron Microscopy Approaches to Study Lipid-Protein Interactions
        2. <b>BIOPHYSICS SEMINAR</b><br>The Versatile Beta-Barrel Gives Up Secrets of the Membrane
        3. <b>BIOPHYSICS SYMPOSIUM</b><br>An International Symposium on Recent Advances in Biomolecular NMR Spectroscopy
        4. <b>BIOPHYSICS SEMINAR</b><br>Hijacking Pathogenic Membrane Proteins to Engineer Cellular Entry: A Molecular Biophysics Approach
        5. <b>BIOPHYSICS SEMINAR</b><br>Sensing of Small Molecules by Natural and in Vitro Evolved RNA Devices
        6. <b>BIOPHYSICS SEMINAR</b><br>Toward Total Synthesis of Chemotaxis, Phagocytosis and Stress Granules
        7. <b>BIOPHYSICS SEMINAR</b><br>Active Matter: Applying the Materials Physics Paradigm to Biology
        8. <b>BIOPHYSICS SEMINAR</b><Br>Advances and Applications in Modern Nuclear Magnetic Resonance: Liquid State Hyperpolarization and Solid State Methods for Amyloid Fibrils
        9. <b>BIOPHYSICS SEMINAR</b><br>Having Fun with Unpaired Electrons: Enhancing the Sensitivity of NMR & Radicals Essential for Life
        10. <b>BIOPHYSICS SEMINAR</b><br>Structure of the HIV-1 5' Leader
        11. <b>BIOPHYSICS SEMINAR</b><br>The Safety Dance: Biophysics of Membrane Protein Misfolding and Disease
        12. <b>BIOPHYSICS SEMINAR</b><br>Tripping Up HCN Channel Regulation Through an Interaction with an Accessory Subunit
        13. <b>BIOPHYSICS SEMINAR</b><br>Cooperation, Cheating, and Collapse of Biological Populations
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        16. <b>BIOPHYSICS SEMINAR</b><br>Motion: the Hallmark of Life, from Marsupials to Molecules
        17. Imaging Mast Cell Activation: from Single Molecules to Synapses
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        19. BIOPHYSICS SEMINAR<br>³Nanoscale Architecture of the Immunological Synapse²
        20. <b>BIOPHYSICS SEMINAR</b><br>Role of Secretory Granule Heterogeneity in the Regulation of Ca2+-Triggered Exocytosis
        21. BIOPHYSICS SEMINAR<br>Biophysical Characterization of Recombinant Type II Cannabinoid Receptor, CB2
        22. BIOPHYSICS SYMPOSIUM<br>Flow Sensing in the Vascular System
        23. BIOPHYSICS SEMINAR<br>Watching Enzymes at Work: NMR for Structural and Functional Studies of Cytochromes P450
        24. BIOPHYSICS SEMINAR<br>Molecular Mechanisms of General Anesthetics; from Lipids to Channels
        25. BIOPHYSICS SEMINAR<br>HIV - 1 Capsid Assembly, Maturation & Host Cell Interactions
        26. BIOPHYSICS SEMINAR<br>Protein Structure Prediction and Protein Design
        27. BIOPHYSICS SEMINAR<br>Tapping the Translation Potential of cAMP-Signalling and Amyloid Inhibition using NMR”
        28. BIOPHYSICS SEMINAR <br> How Do Proteins Scan DNA?
        29. BIOPHYSICS SEMINAR<br>Microbial Resistance to Toxic Fluoride: Structural and Functional Features of Fluoride Channels and Transporters
        30. BIOPHYSICS SEMINAR <br> Krimm Lecture: Illuminating Biology at the Nanoscale with Super-Resolution Imaging
        31. BIOPHYSICS SEMINAR<br>Elucidating Roles of Protein Dynamics in Enzyme Function by Molecular Simulation
        32. BIOPHYSICS SEMINAR<br>Mapping Protein Conformational Ensembles and Folding Pathways with Temperature-Jump Two-Dimensional Infrared Spectroscopy
        33. BIOPHYSICS SEMINAR<br>Entry of Ebola Virus & HIV by Membrane Fusion
        34. BIOPHYSICS SEMINAR <br> RNA-Based Gene Expression and Regulation at Single-Molecule
        35. BIOPHYSICS SEMINAR <br> The Structural Choreography of Cellular Self-Cannibalism
        36. BIOPHYSICS SEMINAR <br> Detection and Manipulation of Single Biomolecular Machines
        37. BIOPHYSICS SEMINAR
        38. BIOPHYSICS SEMINAR<br>Evanescence in Fluorescence Excitation and Emission Microscopy
        39. BIOPHYSICS SEMINAR <br> Imaging Biology at High Spatiotemporal Resolution
        40. BIOPHYSICS SEMINAR <br> The Good, The Bad, and The Ugly: Charges and Their Role in Lipid Phase Separation
        41. BIOPHYSICS SEMINAR<br>Bistability, Trigger Waves, and the Spatial Coordination of Mitosis
        42. BIOPHYSICS SEMINAR<br>Modes of Membrane Disruption By Antimicrobial Compounds
        43. BIOPHYSICS GRADUATE STUDENT SEMINAR<br>Zooming into the Misfolding of the Alzheimer’s Amyloid-ß Peptide
        44. BIOPHYSICS SEMINAR<br>The CH--O Hydrogen Bond - Hiding in Plain Sight
        45. Biophysics Graduation Reception
        46. BIOPHYSICS SEMINAR<br>Insights into Stochastic Growth and Division of Single Bacterial Cells, or A Bug's Life</br>
        47. BIOPHYSICS SEMINAR<br>Structural Determinants and Functional Consequences of Protein Partitioning to Membrane Domains</br>
        48. BIOPHYSICS SEMINAR<br>Looking at Proteins Inside Live Cells with Atomic Resolution: Science Fiction or Science Reality?
        49. BIOPHYSICS SEMINAR<br>Mechanisms of Regulated Protein Degradation During the Bacterial Cell Cycle</br>
        50. BIOPHYSICS SEMINAR<br>Localization-Based Super-Resolution Imaging: Methods and Biological Applications
        51. SPECIAL BIOPHYSICS & COMPLEX SYSTEMS SEMINAR<br>Universal Mechanisms of Multicellular Computation
        52. BIOPHYSICS SEMINAR<br>Exoplanets and Astrobiology</br>
        53. BIOPHYSICS SEMINAR<br>Optical Trapping and Multi-parameter Analysis of Single HIV-1 in Culture Media Reveal the Positive Cooperativity of Envelope Spikes in Mediating Viral Infection
        54. BIOPHYSICS SEMINAR<br>Over the Brainbow – Tracing Neural Circuits in the Mouse Brain
        55. SPECIAL ASTRONOMY & BIOPHYSICS SEMINAR<BR>Exoplanets and Astrobiology
        56. SPECIAL BIOPHYSICS/ASTRONOMY SEMINAR<br>Peering Into the Atmospheres of Extrasolar Worlds</br>
        57. BIOPHYSICS SEMINAR | Structural Gymnastics by Proteins Make the Clock Mechanism Go Around
        58. BIOPHYSICS SEMINAR | NMR of Large Proteins: Molecular Chaperones
        59. BIOPHYSICS SEMINAR | Facile NMR Structure Determination of Human Membrane Proteins
        60. BIOPHYSICS SEMINAR | Cytochrome P450 Steroid Oxidations: Answers and More Questions
        61. BIOPHYSICS SEMINAR | Control of Substrate Access to the Active Site in Methane Monooxygenase
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Friday, November 14, 2014
5:00 AM
1300 Chemistry

Evanescent light (which decays quickly in one spatial dimension) has properties that make it useful (or potentially useful)  for a variety of studies in cell biology.  In fluorescence excitation, evanescence constitutes basis of the familiar TIRF illumination.  The unique polarization properties of TIRF can report on deformations in the cell membrane; examples are given during the dynamic process of secretion.  In fluorescence emission, evanescence constitutes the basis of the fluorophore near-field.  By capturing that near field, images of surface-proximal fluorophores can be recorded.  Point-by-point measurement of near field /far field ratios can provide absolute distances of fluorophores to a surface and in principle, pseudo-images of these distances could be reconstructed by scanning.

Speaker:
Physics
Randall Lab and Homer A. Neal Lab
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