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Biological Mass Spectrometry

About: 

The Biological Mass Spectrometry facility provides specialized MS services to the University of Michigan research community. The two major areas of focus of the lab are: 1) analysis of intact proteins, multi-protein complexes, and protein structure; and 2) identification and purification of compounds from complex mixtures coupled with quantitative analysis. Users can be trained on the instruments for walk-up use or samples can be submitted for analysis. The facility will move into approximately 1000 sq. ft. of newly renovated laboratory space in the spring of 2021.

 

Contact:

Tracy Stevenson 
steventi@umich.edu; 734-647-7316
Chemistry Rm A525,
930 N. University Ave., Ann Arbor, MI, 48109
 

Instruments:

Cyclic IMS Q-TOF (Waters Corporation) In this state-of-the-art mass spectrometer, the traditional linear ion mobility region is replaced with a compact cyclic ion guide, leading to greater ion mobility resolution. This configuration introduces the unique ability to perform ion mobility/ion mobility and IMSn experiments. This system is supported by an M-Class ACQUITY UHPLC liquid chromatograph.

Q Exactive Ultra High Mass Range (UHMR) Orbitrap (Thermo Fisher Scientific) This MS offers high-resolution native intact mass identification (up to 80,000 m/z) and MS/MS capabilities for detailed analysis of protein complex stoichiometries, drug-associated assemblies, and top- and middle-down proteomic analyses.

TSQ Quantis Triple-Stage Quadrupole (Thermo Fisher Scientific) This system is optimized for targeted quantitation of a wide array of molecular species, including those found in complex mixtures, using single reaction monitoring (SRM) or multiple reaction monitoring (MRM) modes of operation. This MS is supported by a Vanquish UHPLC liquid chromatograph.

AutoPurification Preparative Scale LC/MS (Waters Corporation) This system enables high-throughput separation and fractionation of compounds from naturally-occurring sources or from multi-step chemical synthesis strategies. Features of this platform include a SQD2 quadrupole mass spectrometer, photodiode array detector, and AutoPurify to automatically screen samples during processing.