Patricia S. Yaeger Collegiate Professor, MCDB
ujakob@umich.eduOffice Information:
5014 BSB
phone: 734.615.1286
Molecular, Cellular, and Developmental Biology
Education/Degree:
B.S. 1991, Regensburg University, GermanyPh.D. 1995 , Regensburg University
Highlighted Work and Publications
Quantitative In Vivo Redox Sensors Uncover Oxidative Stress as an Early Event in Life:
Daniela Knoefler, Maike Thamsen, Martin Koniczek, Nicholas J. Niemuth, Ann-Kristin Diederich, Ursula Jakob
Obstacles in elucidating the role of oxidative stress in aging include difficulties in (1) tracking in vivo oxidants, in (2) identifying affected proteins, and in (3) correlating changes in oxidant levels with life span. Here, we used quantitative redox proteomics to determine the onset and the cellular targets of oxidative stress during Caenorhabditis elegans' life span. In parallel, we used genetically encoded sensor proteins to determine peroxide levels in live animals in real time. We discovered that C. elegans encounters significant levels of oxidants as early...
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Order out of Disorder: Working Cycle of an Intrinsically Unfolded Chaperone:
Dana Reichmann, Ying Xu, Claudia M. Cremers, Marianne Ilbert, Roni Mittelman, Michael C. Fitzgerald, Ursula Jakob
The redox-regulated chaperone Hsp33 protects organisms against oxidative stress that leads to protein unfolding. Activation of Hsp33 is triggered by the oxidative unfolding of its own redox-sensor domain, making Hsp33 a member of a recently discovered class of chaperones that require partial unfolding for full chaperone activity. Here we address the long-standing question of how chaperones recognize client proteins. We show that Hsp33 uses its own intrinsically disordered regions to discriminate between unfolded and partially structured folding intermediates. Binding to secondary structure...
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